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98804
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MOLECULAR ONCOLOGY, IMMUNOLOGY, AND ENDOCRINOLOGY
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10
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98805 - MOLECULAR ONCOLOGY, IMMUNOLOGY
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MED/04
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5
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5 CFU CARATTERIZZANTI Discipline medico-chirurgiche e della riproduzione umana
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Molecular Oncology
Cancer represents a special type of pathology that can be potentially dangerous even when affecting only an individual cell. At a molecular (biochemical-interactions) level, fundamental cellular functions (proliferation, apoptosis, differentiation, tissue architecture) are controlled by networks of signaling-proteins. Signaling-proteins networks decide when and in which way a cell will activate the above mentioned basic cellular functions. Special discrete sets of mutations / alterations, inheritable at the somatic level, from a parental to a daughter cell, can give origin to new progressively transformed cells, endowed with more permissive controls of these same functions. We want the student to learn the basic features of this pre-neoplastic and subsequently neoplastic transformation process. By about ten years, medicinal chemistry has started to discover selective inhibitors of altered signaling-proteins affected by excess of function (especially kinase inhibitors). We want the student to enter the world of possible selective corrections of altered pathways and altered signaling-network sub-regions.
Molecular Immunology
This course is aimed to provide a broad view of the current knowledge of the molecular mechanisms involved in the antigen-specific immune response and in innate immunity. Examples of translational applications of this basic knowledge to biotechnology approaches of therapy and diagnosis in neoplastic and immune-mediated diseases will be discussed.
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98806 - ENDOCRINOLOGY
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MED/13
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5
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5 CFU CARATTERIZZANTI Discipline medico-chirurgiche e della riproduzione umana
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Elements of clinical and experimental endocrinology. Analytical methods for the evaluation of serum hormonal levels, principles of diagnosis of endocrinological diseases.
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10 CFU tra i seguenti insegnamenti:
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98807
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CELL BIOLOGY APPLIED TO BIOMEDICINE
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BIO/13
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5
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5 CFU A SCELTA A scelta dello studente
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This class will offer a comprehensive view on the current state-of-the-art of regenerative medicine and tissue engineering to the undergraduate students, asking them to examine in depth specific topics in order to develop higher criticism skills.
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102000
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CLINICAL EMBRYOLOGY
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BIO/06
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The advanced course aims to deepen the theoretical and practical aspects of clinical embryology discussing IVF laboratory safety and quality issues, discussing the current practices in IVF and developments in embryology and IVF technology.
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-
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95323
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COMPUTATIONAL TECHNIQUES OF DRUG DEVELOPMENT AND DRUG SYNTHESIS
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CHIM/08
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5
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5 CFU A SCELTA A scelta dello studente
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The course aims to provide key concepts of drug design and synthesis, with particular reference to innovative methodologies.
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98817
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DRUG METABOLISM
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CHIM/09
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The course provides the students with the basic concepts of the metabolism of drugs and other xenobiotics. Besides describing the main Phase 1 and 2 reactions involved, the course deal with the latest in vivo and in vitro technologies used to study drug metabolism, as well as the main enzymatic systems responsible for these biotransformations.
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98815
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GENE THERAPY
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BIO/13
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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To allow the student to acquire a detailed knowledge of the current and up-to date gene transfer and therapy approaches, along with their evolution in clinical settings, and to correlate them with the current knowledge on physiology, molecular biology and genetic engineering technologies; to allow the student to envisage possible future applications of gene therapy based on the current knowledge of monogenic diseases with a relevant clinical prevalence.
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111527
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INSTRUMENTATION FOR PROTEOMICS
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BIO/12
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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From biochemistry to proteomics
The course aims to provide the basis for understanding the main techniques applied for the study of proteins, including:
one-dimensional polyacrylamide gel electrophoresis in native or denatured and denatured reduced and alkylated conditions, zymograms, different types of two-dimensional electrophoresis (i.e. titration curves, blue native electrophoresis, and high-resolution two-dimensional gel electrophoresis), western blots, different methods of proteins detection gels (coomassie, colloidal, silver nitrate and fluorescence staining), main chromatography techniques including methods that use magnetic nano beads, particularly suitable for robotic automation systems, mass spectrometry high resolution, direct and indirect ELISA, membrane and microscope slide protein arrays.
Protein samples preparation
The course aims to provide an overview of the main biochemical-physical methods to address and solve most of the problems in the preparation of protein samples such as most common biological fluids (serum, plasma, liquor and urine), cells, extracellular vesicles and tissues for the methods indicated above.
Bioinformatics analysis
Finally, the course aims to provide the basis for the analysis of big data obtained from protein microarray and/or mass spectrometry experiments. In particular, attention will be paid to: a) how to normalize data, b) the use of the most recent classification, prediction, and correlation algorithms between experimental features and biochemical-clinical data of samples such as weighted gene co-expression network analysis, machine learning and supervised /unsupervised cluster analysis, c) which statistical tests to use, d) how to choose the variables of interest, e) and the gene ontology enrichment analysis for the identification of biological processes and metabolic pathways perturbed in a case-control experiment.
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98814
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MOLECULAR BASES OF THERAPY DESIGN
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MED/04
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The course is aimed at exploring specific example pathologies as consequences of molecular alterations, with particular focus on tumors, and at illustrating novel methodological strategies to investigate disease pathogenetic mechanisms and identify new therapeutic targets.
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95324
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NEUROBIOLOGY AND NEUROPHYSIOLOGY
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BIO/09
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5
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5 CFU A SCELTA A scelta dello studente
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The purpose of the course is to give an overview of molecular mechanisms that regulate functions in the nervous system with special emphasis on cellular and molecular mechanisms that regulate neurotransmission. In particular students receive a detailed information on the generation and propagation of the electrical signal in single neurons up to the complexity of the firing of neurons in network. Mechanisms underlying synaptic plasticity, fundamental for higher function such as learning and memory, will be analyzed in details. Moreover thank to the laboratory-activity, the students will learn basic skills in vitro electrophysiology (extra- and intracellular recordings, patch-clamp) and functional imaging techniques. Different recording modes and their advantages and disadvantages will be dealt with. By the end of the course the students should be able to understand the possibilities and the limitations of the different in vitro experimental approaches in neuroscience research and to design experiments in which the techniques are applied in a relevant manner to deal with up to date neurophysiology questions.
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98820
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NEUROIMMUNOPATHOLOGY
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MED/26
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The aim of the course is to present and discuss the neuropathology of the most common disorders of the central nervous system , such as Parkinson disease and Multiple Sclerosis. Moreover the animal models of multiple sclerosis, experimental allergic encephalomyelitis and of genetic disease of the peripheral nervous system will be presented.
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98818
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NEUROPHARMACOLOGY
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BIO/14
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The aim of the course is to provide the students with knowledge about the main classes of drugs active on the central nervous system. The course will be focused to study the main pharmacological strategies used to modulate the neurochemical and behavioral responses to affect the pathogenetic mechanisms of the main CNS diseases.
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98816
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PHARMACOGENOMICS AND PHARMACOGENETICS
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BIO/14
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2.5
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2.5 CFU A SCELTA A scelta dello studente
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The aim of this course is to provide the students with knowledge about fundamental concepts of Pharmacogenomics and Pharmacogenetics. The course will be focused to study the changes of cellular and molecular mechanisms which regulate the different pharmacological responses. Moreover, the course will underline the most interesting progresses in therapy due to this new potential pharmacological approach and the possible future therapeutic development.
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95322
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PROTEIN EXPRESSION AND ENGINEERING
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BIO/10
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5
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5 CFU A SCELTA A scelta dello studente
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The aim of the course is to provide students with theoretical knowledge but also practical on the assumptions and techniques underlying the engineering of proteins and antibodies. In addition, part of the course will be dedicated to the deepening of the techniques of chemical synthesis of proteins.
Regarding the form of Protein engineering, will initially be resumed and expanded some concettivi base relative to the production of recombinant proteins. Thus, different approaches will be analyzed in order to obtain modified proteins or optimized for different applications, the techniques used and the systems of selection / screening, and using examples from the literature or used for production / commercial.
In the section of the course on the engineering of antibodies will be given with the three-dimensional structure of immunoglobulins and will be addressed later, in the chronological order of creation, the different techniques of humanization and production of recombinant phage display antibody.
In the form of Chemical Engineering explain the main techniques for the chemical synthesis of peptides and proteins and applications resulting from these techniques.
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